BIOPOLIS, S.L. - Agri-food biotechnology
Agri-food biotechnology

BIOPOLIS, S.L. isolates and selects microorganisms, as well as performing the relevant functional and technological validation tests. We also develop and validate functional ingredients for the food industry.

Functional and technological validation trials

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  • Agri-food industry Agri-food industry
  • Pharmaceuticals and cosmetics Pharmaceuticals and cosmetics

BIOPOLIS, S.L. provides services related to the search and evaluation of novel functional ingredients and probiotics.

To be competitive in the food industry, it is essential to validate new ingredients in a fast, reliable and cost-effective way. Therefore BIOPOLIS, S.L. has developed novel high-throughput screening models in vitro and in vivo, which enable us to screen and characterize the target compound and define its metabolic pathway.

We can validate functional ingredients with biological activity against health disorders such as:

  • Aging
  • Oxidative stress
  • Obesity
  • Diabetes
  • Cardiovascular diseases
  • Hypertension
  • Neurodegenerative diseases
  • Infection by pathogens
  • Toxicity
  • Immunity
  • Inflammation

Functional validation methods:

To evaluate enzyme inhibition involved in certain metabolic disorders such as:

  • Obesity, diabetes, hypertension, cardiovascular and neurodegenerative diseases.

To evaluate spoilage microorganisms and/or pathogen growth inhibition.

BIOPOLIS, S.L. has developed novel high-throughput screening models in vivo, which provide fast screening of the target compound and definition of its metabolic pathway.

Saccharomyces cerevisiae

S. cerevisiae is one of the most intensively studied eukaryotic model organisms in molecular and cell biology due to its ease of handling and because it shares the complex internal cell structure of plants and animals.

Caenorhabditis elegans (ver pdf)

C. elegans is a high-throughput model to study the functional effects of nutraceuticals and novel food ingredients as well as foods against certain disorders:

  • Aging (antioxidant capacity): C. elegans is an excellent model to study aging due to its short lifespan, the ease of inducing oxidative stress and similarity with human aging processes.
  • Obesity: many genes involved in the fat-regulatory pathways of C. elegans are conserved in humans, and they are involved in appetite, intestinal function and metabolism.
  • Diabetes: in C. elegans an insulin-like signalling pathway culminates in a transcription factor that is homologous in humans and regulates metabolism, development and longevity.
  • Alzheimer’s disease: there is a transgenic worm expressing the human Aβ42 gene. These “sick” worms form amyloid aggregates of different sizes, like those found in brains affected by Alzheimer’s.
  • Microbial infections: C. elegans is used as a simple and traceable host model for infectious diseases.

This in vivo model along with nutrigenomics defines compound functionalities and metabolic targets very accurately and quickly. Nutrigenomics is a new technology that provides insight into how functional ingredients modulate the gene expression regulating a specific metabolism. C. elegans is fed on the selected ingredient and then gene expression is analyzed to determine the nutrient-gene interaction or the metabolic target of this specific ingredient.

Murine models (rat/mouse)

Prior to clinical studies, assays in murine models are one of the most useful approaches in order to ensure functional food properties. Functional murine trials:

  • Efficacy assays (immune-response ).
  • Efficacy assays (in vivo activity against human and animal pathogens).
  • Safety trials (acute ingestion assays)

Ex vivo: cell culture

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In this procedure, living cells or tissues taken from an organism are cultured to screen the biological activity of a specific compound to substitute or replace existing in vivo models.

A wide range of cell cultures can be employed, depending on the activity to be measured and customer needs.