BIOPOLIS, S.L. - Microbial Biotechnology
Microbial Biotechnology

We select, design and improve microbial strains and bioprocesses for new product development and for novel ways of producing organic compounds for industry.

Biocatalytic process set up and optimization

  • Pharmaceuticals and cosmetics Pharmaceuticals and cosmetics
  • Energy Energy
  • Environment Environment
  • Chemical industry Chemical industry
  • Other Applications Other Applications

We develop processes for the enzymatic conversion of selected substrates into high added-value biochemical compounds. To do so, commercial enzyme preparations can be used or specifically designed biocatalysts, by enzymes or permeabilized cell concentrates.

  • Stereo and enantioselective synthesis by esterification, epoxidation, hydroxylation, phosphorylation, methylation, transglycosylation, sulfation of various substrates.
  • Identification of target enzyme activity by metagenomics and metatranscriptomics.
  • Expertise in desaturases, hydrolases, laccases, lipases, monooxygenases, kinases, nucleotidyl-transferases, peroxidases.
  • Definition of enzymatic synthesis reactions: substrate ratio, solvent selection, determination of kinetic constants and optimization of ambient environmental conditions (temperature, pH).
  • Immobilization of enzymes for their stabilization and reuse, modification of properties by adsorption, covalent immobilization or crosslinking.
  • Conversion by whole-cell preparations: design of genetically modified microorganisms, selection of permeabilizing agents, stability, process optimization (temperature, pH, oxygen concentration).
  • Definition of suitable analytical methods for specific determination and monitoring (thin layer chromatography, spectrophotometry, fluorescence and chemiluminescence, GC, HPLC).
  • Testing in bioreactors at scalable volumes (1 ml to 1 m3).
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