BIOPOLIS, S.L. - Microbial Biotechnology
Microbial Biotechnology

We select, design and improve microbial strains and bioprocesses for new product development and for novel ways of producing organic compounds for industry.

Screening and development of cell lines

  • Pharmaceuticals and cosmetics Pharmaceuticals and cosmetics
  • Agri-food industry Agri-food industry
  • Other Applications Other Applications

At the BIOPOLIS, S.L. cell-culture laboratory, our highly-qualified personnel carry out projects to develop and select cell cultures, to obtain recombinant proteins and/or perform assays with the cell lines developed.

Development and selection of mammalian cell lines

  • Subcloning of the gene of interest into a suitable expression vector for mammalian cells.
  • Gene synthesis with adapted codon usage where necessary.
  • Epitope tagging to facilitate protein detection and/or purification.
  • Assay for transient expression of the gene of interest, optimal time and level of expression.
  • Selection of cell lines with stable expression.
  • Adapting selected cell lines to serum-free growth.
  • Evaluation of expression stability.
  • Cryopreservation of cell lines.
  • Quality control of the lines generated (guaranteed mycoplasma free).

Recombinant protein expression in insect cells

  • Subcloning into suitable expression vectors.
  • Generation of high-titre baculovirus stock.
  • Expression analysis.
  • Optimization of infectivity parameters.

Production of monoclonal antibodies.

  • Hybridoma cell storage and conservation.
  • Acquisition of cell culture supernatants of high antibody titer.
  • Monoclonal antibody purification using affinity chromatography.
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